Knowledge required to create a biosphere from scratch
Establish the scientific and engineering knowledge needed to construct a self-sustaining biosphere on Mars de novo, including identification and engineering of pioneer organisms, biomaterials production, habitat replication, pedogenesis, biogeochemical feedbacks, and ecosystem function under Martian environmental conditions.
References
We do not yet know enough to create a biosphere from scratch.
— A research roadmap for assessing the feasibility of warming Mars
(2604.02242 - Kite et al., 2 Apr 2026) in Section 1, Introduction