Quantify the number of true ac4C sites represented in the positive-sample sets

Ascertain the exact number of true N4-acetylcytidine (ac4C) modification sites present within the collected positive-sample sets derived from acRIP-seq peak and sub-peak data for mRNA, recognizing that these sets contain true ac4C sites but the total quantity is currently unknown.

Background

The study constructs positive-sample sets from acRIP-seq peak and sub-peak regions, which are known to include true ac4C modifications but lack base-level resolution. As a result, the dataset includes true signals without a clear count of how many distinct ac4C sites are represented.

This uncertainty complicates evaluation, validation, and interpretation of computational models and motif analyses, motivating a need to quantify the exact number of true ac4C sites encompassed by the positive sets.