Temporal scaling of the growth dependent radiative properties of microalgae

Abstract: The radiative properties of microalgae are basic parameters for analyzing light field distribution in photobioreactors (PBRs). With the growth of microalgae cell, their radiative properties will vary with growth time due to accumulation of pigment and lipid, cell division and metabolism. In this work, we report both experimental and theoretical evidence of temporal scaling behavior of the growth dependent radiative properties of microalgae cell suspensions. A new concept, the temporal scaling function (TSF), defined as the ratio of absorption or scattering cross-sections at growth phase to that at stationary phase, is introduced to characterize the temporal scaling. The temporal evolution and temporal scaling characteristics of the absoroption and scattering cross-sections of three example microalgae species, Chlorella vulgaris, Chlorella pyrenoidosa, and Chlorella protothecoides, were experimentally studied at spectral range 380 to 850 nm. It is shown that the TSFs of the absorption and scattering cross-sections for different microalgae species are approximately constant at different wavelength, which confirms theoretical predictions very well. With the aid of the temporal scaling relation, the radiative properties of microalgae at any growth time can be calculated based on those measured at stationary phase. The findings of this work will help the understanding of time dependent radiative properties of microalgae and facilitate their applications in light field analysis in PBRs design.