Epithelial-substrate coupling strength regulates the landscape of the traction in cohesive monolayers: a parametric study and a revisit to "size effect"

Abstract: Epithelial cells can assemble into cohesive colonies and collectively interact with substrates by generating extracellular forces through focal adhesions. Recently, a molecularly based thermodynamic model, which integrates both the monolayer elasticity and force-mediated focal adhesion formation, has been developed to elucidate the regulation of the cellular force landscape induced by the active epithelial-substrate coupling. However, how epithelial-substrate coupling strength mediate the landscapes of the traction, the cellular displacement, and the focal adhesion distribution in a cohesive monolayer remains unexamined in details. In this work, we follow the procedures by the previous work to re-formulate the free energy of the epithelial-substrate system and obtain the thermodynamic steady-state equations. We then derive a simplified form of the complete equation system, and solve it both semi-analytically and numerically. We find that the parameter which characterizes the epithelial-substrate coupling strength can significantly affect the landscapes of the traction the cellular displacement, and the focal adhesion distribution. We also revisit the "size effect" addressed by previous works and demonstrate that such effect is the natural outcome of a strong epithelial-substrate coupling without introducing any extra factors. For epithelial-substrate coupling which is not strong enough, the currently observed "size effect" does not hold. A scaling law that determines whether the previously observed "size effect" holds is proposed based on our model.