Dynamic structured illumination for confocal microscopy

Abstract: Structured illumination enables the tailoring of an imaging device's optical transfer function to enhance resolution. We propose the incorporation of a temporal periodic modulation, specifically a rotating mask, to encode multiple transfer functions in the temporal domain. This approach is demonstrated using a confocal microscope configuration. At each scanning position, a temporal periodic signal is recorded. By filtering around each harmonic of the rotation frequency, multiple images of the same object can be constructed. The image carried by the $n{\mathrm{th}}$ harmonic is a convolution of the object with a phase vortex of topological charge $n$, similar to the outcome when using a vortex phase plate as an illumination. This enables the collection of chosen high spatial frequencies from the sample, thereby enhancing the spatial resolution of the confocal microscope.