MinD the gap: Membrane proteins form 3D patterns in a suspension of liposomes

Abstract: The self-organization of pattern-forming systems depends not only on the chemical but also physical properties of their components. In this work, we fragmented and dispersed the MinDE protein system's lipid substrate into diffusive sub-micrometer-sized liposomes, and report that the ATP-fueled protein-protein interactions continue to drive spatially extended patterns at scales well separated from those of the requisite liposomes, despite the complete loss of membrane continuity. The patterns form in three-dimensions because the membrane is dispersed in a volume. By varying protein concentration, liposome size distribution, and density, we observed and characterized rich 3D dynamical patterns at steady state, including traveling waves, dynamical spirals and a mixed phase where both patterns coexist. Simulations and linear stability analysis of a coarse-grained model reveal that the dispersed membranes's physical properties effectively rescale two key factors that govern pattern formation and wavelength selection: protein-membrane binding rates and diffusion. This work highlights the robustness of pattern formation in membrane-bulk systems despite membrane fragmentation. It suggests that biological protein systems have the potential to serve as adaptable templates for out-of-equilibrium self-organization in 3D, beyond in vivo biological contexts.