Lattice and Orientational Defects Mediate Collective Transport of Confluent Cells

Abstract: Confluent tissues are a type of foam-like biological active matter. There is a recent interest in using active nematic liquid crystal framework to understand confluent cells, where topological (orientational) defects are believed to play a crucial role. However, how to reconcile the physical picture of lattice defects in Voronoi polygons with that of orientational defects in the nematic field$-$particularly in the context of cellular transport$-$remains elusive. Here, we employ the Active Vertex model to investigate the physics of lattice and orientational defects in the dynamics of confluent cells. We find a spatio-orientational correlation between lattice defects and $+1/2$ defects, unveiling a correspondence between the two physical perspectives. Next, we simulate the behavior of a dragged cell within a hexagonal-lattice tissue. Our results reveal that while the drag coefficient is anisotropic, the threshold drag force to mobilize the cell is isotropic, indicating the presence of a caging energy barrier. We further analyze the defect pattern in the wake of the dragged cell or cells. Remarkably, we discover that dragging two neighboring cells along the least-drag direction can substantially minimize the destruction of the lattice structure during cell transport. We find that this is due to the cooperative and periodic self-healing of the lattice defects. Taken together, our work sheds new light on the topological structure of confluent cells during their collective motion, advancing our physical understanding of cellular transport in processes such as wound healing, cancer cell metastasis, and other physiological events.