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Characterisation of the signal to noise ratio of 2-photon microscopes

Published 24 Jul 2025 in physics.optics and physics.ins-det | (2507.18191v1)

Abstract: Signal to noise ratio (SNR) is a key performance metric of an imaging instrument. Here we describe characterisation of SNR of a custom 2-photon microscope and compare the SNR performance of our microscope with selected commercial 2-photon microscopes. The methodology described in this paper can serve as guidance for others wishing to characterise and benchmark their 2-photon or other point-scanning microscopes.

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