Field‑relevant validation of biomolecular controllers

Demonstrate and evaluate biomolecular feedback and feedforward controllers for gene expression in realistic biological contexts beyond laboratory cell lines and strains, moving toward field‑relevant applications and use cases.

Background

Most experimental validations to date have been performed in controlled laboratory settings and in standard mammalian or bacterial lines. Translating these controllers to more complex or clinically relevant environments is essential to assess robustness and utility.

The paper presents iPSC reprogramming as an initial example but emphasizes the need for broader, application‑proximate testing.