Required imaging resolution and molecular marker diversity for predictive performance

Determine the minimum spatial resolution and the breadth of molecular marker diversity in molecularly annotated ultrastructural imaging that are necessary to achieve accurate inference of synaptic and compartmental parameters by an ultrastructure-to-dynamics compiler.

Background

The authors emphasize that protein counts and presence do not directly translate to effective conductances or kinetics, as trafficking, subunit assembly, and nanoscale organization can be critical.

Identifying the spatial resolution and the set of molecular labels needed to capture functionally relevant organization is a central unknown that constrains the design and feasibility of training datasets for the compiler.